home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410p.zip
/
M94A3312.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
3KB
|
45 lines
Document 3312
DOCN M94A3312
TI A new method of selecting HIV inhibitor in vitro.
DT 9412
AU Cai C; Koga Y; Nomoto K; Medical Institute of Bioregulation, Kyushu
University, Fukuoka,; Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):105 (abstract no. PA0038). Unique
Identifier : AIDSLINE ICA10/94369263
AB OBJECTIVE: A safe and rapid method of selecting HIV inhibitor in vitro
is necessary for AIDS research and therapy. We established the method on
HIV-1 env gene expression cell lines. METHODS: Human CD4+ T and
monocytoid cell lines were transferred with HIV-1 env gene expression
vector in which the activity of the promotor could be augmented 10-20
times by heavy ions. Each cell population adjusted to 100,000 cells in 1
ml RPMI 1640 medium containing 0 or 20 microM cadmium chloride was
cultured with dextran sulphate (MW 5,000, 0 or 100 micrograms/ml),
mitomycin (0 or 1 ng/ml), alpha-amanitin (0 or 1 microgram/ml) or
cycloheximide (0 or 1 microgram/ml) respectively. The cell numbers were
determined every second day. RESULTS: When the expression of HIV-1 env
gene was induced by heavy ions, the cell line derived from CD4+ T cell
showed extensive cytopathic effects, such as syncytium formation (12,000
giant cells/ml/day 6) and cell death (a 49.8% cell death in a total of
2,170,000 cells/ml/day 6); the cell line derived from CD4+ monocytoid
cell also showed cell death (a 528% cell death in a total of 2,180,000
cells/ml/day 6) but syncytium formation. After dextran sulphate was
administered, both cell lines showed no syncytium formation and low
death rates (a 0.9% cell death in the total of 2,170,000 T cells/ml/day
6; a 5.0% cell death in the total of 2,180,000 monocytoid cells/ml/day
6). The administrations of mitomycin, alpha--amanitin or cycloheximide
failed to show any significant cytopathic inhibitory effects. DISCUSSION
AND CONCLUSIONS: Dextran sulphate blocks the cell fusion and protects
the cell from death on HIV-1 env gene expressing CD4+ T and monocytiod
cells. The method is recommendable as a safe and rapid method of
selecting HIV inhibitor in vitro.
DE Antiviral Agents/*PHARMACOLOGY Cell Death/DRUG EFFECTS Cell
Fusion/DRUG EFFECTS Cell Line Cytopathogenic Effect, Viral/DRUG
EFFECTS Dextran Sulfate/PHARMACOLOGY Drug Screening/*METHODS Gene
Expression/DRUG EFFECTS Genes, env/DRUG EFFECTS Human HIV-1/*DRUG
EFFECTS/GENETICS/PATHOGENICITY Monocytes/DRUG EFFECTS/MICROBIOLOGY T4
Lymphocytes/DRUG EFFECTS/MICROBIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).